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人丝状肌动蛋白(F-actin)酶联免疫吸附检测试剂盒

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ELK0113

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human F-actin(Filamentous Actin) ELISA Kit
Reactivity:	Human
Alternative Names:	Fibrous Actin
Assay Type:	Sandwich
Sensitivity:	0.18 ng/mL
Standard:	40 ng/mL
Detection Range:	0.63-40 ng/mL
Sample type:	Serum, plasma, tissue homogenates
Assay length:	3.5h
Research Area:	Signal transduction;Developmental science;Bone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
40.00	2.532	2.456
20.00	1.685	1.609
10.00	1.124	1.048
5.00	0.749	0.673
2.50	0.593	0.517
1.25	0.385	0.309
0.63	0.211	0.135
0.00	0.076	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant F-actin and the recovery rates were calculated by comparing the measured value to the expected amount of F-actin in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	80-93%	86%
Heparin plasma(n=5)	94-105%	98%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-actin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.