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大鼠固醇调节元件结合蛋白1c(SREBP-1c)酶联免疫吸附检测试剂盒

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ELK0117

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit
Reactivity:	Rat
Alternative Names:	ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1
Assay Type:	Sandwich
Sensitivity:	18.3 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SREBP-1C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SREBP-1C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SREBP-1C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SREBP-1C in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
4000.00	2.168	2.080
2000.00	1.569	1.481
1000.00	1.217	1.129
500.00	0.937	0.849
250.00	0.566	0.478
125.00	0.315	0.227
62.50	0.175	0.087
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant SREBP-1C and the recovery rates were calculated by comparing the measured value to the expected amount of SREBP-1C in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA plasma(n=5)	97-107%	102%
Heparin plasma(n=5)	87-99%	93%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SREBP-1C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.