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人糖类抗原15-3(CA15-3)酶联免疫吸附检测试剂盒

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ELK0245

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit
Reactivity:	Human
Alternative Names:	CA15-3
Assay Type:	Sandwich
Sensitivity:	0.47 U/mL
Standard:	100 U/mL
Detection Range:	1.57-100 U/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (U/mL)	OD	Corrected OD
100.00	2.126	2.040
50.00	1.613	1.527
25.00	1.226	1.140
12.50	0.826	0.740
6.25	0.576	0.490
3.13	0.373	0.287
1.57	0.135	0.049
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CA15-3 and the recovery rates were calculated by comparing the measured value to the expected amount of CA15-3 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	88-106%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA15-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.