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人抗泛素结合蛋白P62抗体(Anti-p62)酶联免疫吸附检测试剂盒

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ELK0521

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human Anti-p62(Ubiquitin Binding Protein P62 Antibody) ELISA Kit
Reactivity:	Human
Alternative Names:	anti-p62; anyi-SQSTM1
Assay Type:	Sandwich
Sensitivity:	0.049 ng/mL
Standard:	8 ng/mL
Detection Range:	0.13-8 ng/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Signal transduction;Developmental science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-p62. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-p62. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-p62, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-p62 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
8.00	2.136	2.047
4.00	1.528	1.439
2.00	1.107	1.018
1.00	0.827	0.738
0.50	0.521	0.432
0.25	0.373	0.284
0.13	0.206	0.117
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-p62 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-p62 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	87-99%	93%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-p62 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.