99499威尼斯(中国·WWW认证)游戏内容-Macau App Station

产品中心

人6酮前列腺素F1α(6-K-PGF1α)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK0571

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human 6-K-PGF1α(6-keto-PGF1 alpha) ELISA Kit
Reactivity:	Human
Alternative Names:	6-keto-PGF1 alpha; 6ketoPGF1α
Assay Type:	Competitive Inhibition
Sensitivity:	5.4 pg/mL
Standard:	800 pg/mL
Detection Range:	12.5-800 pg/mL
Sample type:	Serum, plasma and other biological fluids.
Assay length:	2.5h
Research Area:	Neuro science;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 6-K-PGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 6-K-PGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 6-K-PGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
800.00	0.199
400.00	0.397
200.00	0.722
100.00	0.833
50.00	1.169
25.00	1.585
12.50	1.762
0.00	2.256

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 6-K-PGF1α and the recovery rates were calculated by comparing the measured value to the expected amount of 6-K-PGF1α in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	84-96%	90%
Heparin plasma(n=5)	80-95%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 6-K-PGF1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.