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人抗组织谷氨酰胺转移酶抗体IgG(Anti-tTG IgG)酶联免疫吸附检测试剂盒

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ELK0751

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit
Reactivity:	Human
Alternative Names:	Anti-Tissue Transglutaminase IgG
Assay Type:	Sandwich
Sensitivity:	0.6 ng/mL
Standard:	100 ng/mL
Detection Range:	1.56-100 ng/mL
Sample type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay length:	3.5h
Research Area:	Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	2.035	1.940
50.00	1.663	1.568
25.00	1.121	1.026
12.50	0.815	0.720
6.25	0.585	0.490
3.13	0.549	0.454
1.57	0.203	0.108
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-tTG IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-tTG IgG in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	83-95%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-tTG IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.