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人抗组织谷氨酰胺转移酶抗体IgA(Anti-tTG-IgA)酶联免疫吸附检测试剂盒

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ELK0752

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(1)

Product name:	Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit
Reactivity:	Human
Alternative Names:	Anti-Tissue Transglutaminase IgA
Assay Type:	Sandwich
Sensitivity:	1.45 IU/mL
Standard:	300 IU/mL
Detection Range:	4.69-300 IU/mL
Sample type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay length:	3.5h
Research Area:	Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

SENSORS AND ACTUATORS B-CHEMICAL

Celiac disease biomarker quantification in human fluid microenvironment: A selective and ultrasensitive magnetosensing immunoplatform

日期：2025-03-17 IF：8

标准曲线

Concentration (IU/mL)	OD	Corrected OD
300.00	2.075	1.988
150.00	1.472	1.385
75.00	1.069	0.982
37.50	0.728	0.641
18.75	0.535	0.448
9.38	0.303	0.216
4.69	0.191	0.104
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-tTG-IgA and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-tTG-IgA in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	82-97%	89%
Heparin plasma(n=5)	87-95%	91%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-tTG-IgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.