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人L-甲基叶酸(5-MTHF)酶联免疫吸附检测试剂盒

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ELK0833

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human 5-MTHF(5-Methyltetrahydrofolate) ELISA Kit
Reactivity:	Human
Alternative Names:	L-5-MTHF; 5-MTHF;
Assay Type:	Sandwich
Sensitivity:	0.059 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length:	3.5h
Research Area:	Signal transduction;Reproductive science;Genetic science;Nutrition metabolism;
Uniprot ID:	P49914
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5-MTHF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-MTHF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5-MTHF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5-MTHF in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.041	1.954
5.00	1.634	1.547
2.50	1.143	1.056
1.25	0.827	0.740
0.63	0.511	0.424
0.32	0.357	0.270
0.16	0.206	0.119
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 5-MTHF and the recovery rates were calculated by comparing the measured value to the expected amount of 5-MTHF in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	81-93%	87%
Heparin plasma(n=5)	82-94%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-MTHF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.