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人叶酸受体自身抗体(Anti-FOLR)酶联免疫吸附检测试剂盒

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ELK0865

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human Anti-FOLR(folate receptor autoantibodies IgG) ELISA Kit
Reactivity:	Human
Alternative Names:	ANTI-FOLR1
Assay Type:	Sandwich
Sensitivity:	3.3 ng/mL
Standard:	500 ng/mL
Detection Range:	7.82-500 ng/mL
Sample type:	tissue homogenates, cell lysates and other biological fluids
Assay length:	3.5h
Research Area:	Signal transduction
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-FOLR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-FOLR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-FOLR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-FOLR in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
500.00	2.045	1.962
250.00	1.502	1.419
125.00	1.142	1.059
62.50	0.671	0.588
31.25	0.485	0.402
15.63	0.313	0.230
7.82	0.213	0.130
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-FOLR and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-FOLR in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	83-99%	95%
Heparin plasma(n=5)	90-105%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-FOLR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.