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一步法人甲胎蛋白(aFP)酶联免疫吸附检测试剂盒

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ELK091ES

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	EasyStep Human aFP(Alpha-Fetoprotein) ELISA Kit
Reactivity:	Human
Alternative Names:	A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
Assay Type:	Sandwich
Sensitivity:	0.175 ng/mL
Standard:	100 ng/mL
Detection Range:	1.56-100 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Tumor immunity;Hepatology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aFP, and the Human aFP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human aFP. After TMB substrate solution is added, only those wells that contain Human aFP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	2.027	1.930
50.00	1.576	1.479
25.00	1.106	1.009
12.50	0.836	0.739
6.25	0.457	0.360
3.13	0.325	0.228
1.57	0.226	0.129
0.00	0.097	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant aFP and the recovery rates were calculated by comparing the measured value to the expected amount of aFP in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-93%	85%
Heparin plasma(n=5)	85-97%	91%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of aFP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.