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一步法人可溶性转铁蛋白受体(sTFR)酶联免疫吸附检测试剂盒

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ELK092ES

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	EasyStep Human sTFR(Soluble Transferrin Receptor) ELISA Kit
Reactivity:	Human
Alternative Names:	CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1
Assay Type:	Sandwich
Sensitivity:	17.55 ng/mL
Standard:	5000 ng/mL
Detection Range:	78.13-5000 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTFR, and the Human sTFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human sTFR. After TMB substrate solution is added, only those wells that contain Human sTFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTFR in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
5000.00	2.266	2.181
2500.00	1.563	1.478
1250.00	1.128	1.043
625.00	0.875	0.790
312.50	0.557	0.472
156.25	0.305	0.220
78.13	0.161	0.076
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant sTFR and the recovery rates were calculated by comparing the measured value to the expected amount of sTFR in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	78-95%	86%
Heparin plasma(n=5)	80-96%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTFR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.