99499威尼斯(中国·WWW认证)游戏内容-Macau App Station

产品中心

犬胱抑素C(Cys-C)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK10127

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Dog Cys-C(Cystatin C) ELISA Kit
Reactivity:	Dog
Alternative Names:	CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type:	Sandwich
Sensitivity:	0.64 ng/mL
Standard:	100 ng/mL
Detection Range:	1.56-100 ng/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Tags & Cell Markers；Cell Biology；Stem Cells；Cardiovascular
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	2.132	2.045
50.00	1.624	1.537
25.00	1.223	1.136
12.50	0.933	0.846
6.25	0.593	0.506
3.13	0.357	0.270
1.57	0.189	0.102
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	94-107%	100%
Heparin plasma(n=5)	94-105%	98%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.