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人野鼠色基因相关蛋白(AGRP)酶联免疫吸附检测试剂盒

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ELK10335

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human AGRP(Agouti Related Protein) ELISA Kit
Reactivity:	Human
Alternative Names:	AGRT; ASIP2; ART; Agouti-Related Peptide
Assay Type:	Sandwich
Sensitivity:	5.4 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	Serum, plasma and other biological fluids.
Assay length:	3.5h
Research Area:	Signal transduction;Metabolic pathway;Neuro science;Hormone metabolism;
Uniprot ID:	O00253
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGRP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.268	2.179
500.00	1.642	1.553
250.00	1.109	1.020
125.00	0.882	0.793
62.50	0.548	0.459
31.25	0.322	0.233
15.63	0.196	0.107
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant AGRP and the recovery rates were calculated by comparing the measured value to the expected amount of AGRP in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	78-95%	86%
Heparin plasma(n=5)	80-96%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.