99499威尼斯(中国·WWW认证)游戏内容-Macau App Station

产品中心

人促肾上皮质激素释放激素(CRH)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK10412

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview 文献(1)

Product name:	Human CRH(Corticotropin Releasing Hormone) ELISA Kit
Reactivity:	Human
Alternative Names:	CRF; Corticotropin Releasing Factor; Corticoliberin
Assay Type:	Competitive Inhibition
Sensitivity:	0.13 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;
Uniprot ID:	P06850
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Experimental Neurology

CRHR1 antagonist alleviated depression-like behavior by downregulating p62 in a rat model of post-stroke depression

日期：2024-07-25 IF：4.6

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	0.189
10.00	0.357
5.00	0.575
2.50	0.878
1.25	1.169
0.63	1.537
0.32	1.793
0.00	2.226

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CRH and the recovery rates were calculated by comparing the measured value to the expected amount of CRH in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	88-102%	95%
Heparin plasma(n=5)	80-92%	86%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.