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ELK10889

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview 文献(1)

Product name:	Human p-cresol ELISA Kit
Reactivity:	Human
Alternative Names:	4-Hydroxytoluene; 4-Methylphenol; p-Cresol 98+ %; PARA-CRESOL; 4-cresol; para cresol
Assay Type:	Sandwich
Sensitivity:	4.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-cresol. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve.

Medicina

The Effect of Synbiotic Supplementation on Uremic Toxins, Oxidative Stress, and Inflammation in Hemodialysis Patients—Results of an Uncontrolled Prospective Single-Arm Study

日期：2024-01-12 IF：

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.131	2.048
500.00	1.647	1.564
250.00	1.174	1.091
125.00	0.728	0.645
62.50	0.518	0.435
31.25	0.262	0.179
15.63	0.175	0.092
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant p-cresol and the recovery rates were calculated by comparing the measured value to the expected amount of p-cresol in samples.

Matrix	Recovery range	Average
serum(n=5)	78-94%	86%
EDTA plasma(n=5)	90-103%	96%
Heparin plasma(n=5)	81-96%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-cresol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.