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人8-羟基脱氧鸟苷(8-OHdG)酶联免疫吸附检测试剂盒

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ELK10908

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(4)

Product name:	Human 8-OHdG(DNA Damage) ELISA Kit
Reactivity:	Human
Alternative Names:	8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine
Assay Type:	Competitive Inhibition
Sensitivity:	0.59 ng/mL
Standard:	100 ng/mL
Detection Range:	1.56-100 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

Environmental Research

Evaluation of genotoxicity, oxidative stress and immune parameters of auto-paint workers

日期：2024-04-28 IF：8.3
Toxics

Biomonitoring of Oxidative-Stress-Related Genotoxic Damage in Patients with End-Stage Renal Disease

日期：2024-01-12 IF：4.6
Metabolic Brain Disease

Neuroprotective effects of Saxagliptin in traumatic brain injury: ameliorating oxidative stress, neuroinflammation, and apoptosis

日期：2025-05-13 IF：3.2
Indian Journal of Clinical Biochemistry

Elevated Oxidized LDL Level is Associated with Systematic Coronary Risk Evaluation (SCORE2) High-Risk Prediction Algorithm: A Preliminary Study

日期：2024-06-26 IF：1.5

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	0.191
50.00	0.367
25.00	0.596
12.50	0.861
6.25	1.157
3.13	1.585
1.57	1.775
0.00	2.123

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	80-93%	86%
Heparin plasma(n=5)	94-105%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.