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小鼠上皮型钙黏蛋白(E-Cad)酶联免疫吸附检测试剂盒

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ELK11211

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Mouse E-Cad(E-Cadherin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin
Assay Type:	Sandwich
Sensitivity:	0.132 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Signal Transduction;Cancer;Developmental Biology
Uniprot ID:	P09803
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.179	2.091
10.00	1.607	1.519
5.00	1.140	1.052
2.50	0.852	0.764
1.25	0.527	0.439
0.63	0.305	0.217
0.32	0.252	0.164
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.

Matrix	Recovery range	Average
serum(n=5)	84-96%	90%
EDTA plasma(n=5)	96-107%	101%
Heparin plasma(n=5)	83-95%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.