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人微管关联蛋白2(MAP2)酶联免疫吸附检测试剂盒

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ELK1885

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview 文献(1)

Product name:	Human MAP2(Microtubule Associated Protein 2) ELISA Kit
Reactivity:	Human
Alternative Names:	MAP2A; MAP2B; MAP2C
Assay Type:	Sandwich
Sensitivity:	6.5 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	serum, plasma, tissue homogenates and other biological fluids
Assay length:	3.5h
Research Area:	Neuro science;Developmental science;
Uniprot ID:	P11137
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

BIOCHEMISTRY-MOSCOW

RNA Editing by ADAR Adenosine Deaminases in the Cell Models of CAG Repeat Expansion Diseases: Significant Effect of Differentiation from Stem Cells into Brain Organoids in the Absence of Substantial Influence of CAG Repeats on the Level of Editing

日期：2024-08-30 IF：2.3

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.268	2.179
500.00	1.720	1.631
250.00	1.136	1.047
125.00	0.828	0.739
62.50	0.538	0.449
31.25	0.315	0.226
15.63	0.206	0.117
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MAP2 and the recovery rates were calculated by comparing the measured value to the expected amount of MAP2 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-90%	84%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	78-92%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.