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人血管生成素4(ANGPT4)酶联免疫吸附检测试剂盒

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ELK2120

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human ANGPT4(Angiopoietin 4) ELISA Kit
Reactivity:	Human
Alternative Names:	ANG4; AGP4; ANG-3
Assay Type:	Sandwich
Sensitivity:	7.89 pg/mL
Standard:	1200 pg/mL
Detection Range:	18.75-1200 pg/mL
Sample type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay length:	3.5h
Research Area:	Tumor immunity;Cardiovascular biology;
Uniprot ID:	Q9Y264
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANGPT4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ANGPT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ANGPT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANGPT4 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1200.00	2.266	2.185
600.00	1.497	1.416
300.00	1.073	0.992
150.00	0.839	0.758
75.00	0.529	0.448
37.50	0.356	0.275
18.75	0.187	0.106
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ANGPT4 and the recovery rates were calculated by comparing the measured value to the expected amount of ANGPT4 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	80-93%	86%
Heparin plasma(n=5)	80-95%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANGPT4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.