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大鼠白介素12 p40(IL-12p40)酶联免疫吸附检测试剂盒

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ELK2509

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview 文献(1)

Product name:	Rat IL-12p40(Interleukin 12 p40) ELISA Kit
Reactivity:	Rat
Alternative Names:	IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit
Assay Type:	Sandwich
Sensitivity:	5.6 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Cytokine;Infection immunity;Immune molecule;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Bioactive Materials

Minimally invasive bioprinting for in situ liver regeneration

日期：2023-03-11 IF：18.9

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.056	1.960
500.00	1.527	1.431
250.00	1.135	1.039
125.00	0.752	0.656
62.50	0.563	0.467
31.25	0.332	0.236
15.63	0.175	0.079
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL-12p40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12p40 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	90-104%	97%
Heparin plasma(n=5)	80-97%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12p40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.