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人碱性鞘磷脂磷酸二酯酶(Alk-Smase)酶联免疫吸附检测试剂盒

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ELK2631

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
Reactivity:	Human
Alternative Names:	ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase
Assay Type:	Sandwich
Sensitivity:	0.109 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	tissue homogenates, cell lysates and other biological fluids
Assay length:	3.5h
Research Area:	Enzyme & Kinase;Neuro science;
Uniprot ID:	Q6UWV6
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.096	2.014
10.00	1.602	1.520
5.00	1.067	0.985
2.50	0.872	0.790
1.25	0.572	0.490
0.63	0.302	0.220
0.32	0.165	0.083
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Alk-Smase and the recovery rates were calculated by comparing the measured value to the expected amount of Alk-Smase in samples.

Matrix	Recovery range	Average
serum(n=5)	84-106%	100%
EDTA plasma(n=5)	87-93%	90%
Heparin plasma(n=5)	80-90%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alk-Smase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.