99499威尼斯(中国·WWW认证)游戏内容-Macau App Station

产品中心

人前动力蛋白2(PK2)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK2816

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human PK2(Prokineticin 2) ELISA Kit
Reactivity:	Human
Alternative Names:	PK-2; Bv8; MIT1; KAL4; Protein Bv8 Homolog
Assay Type:	Competitive Inhibition
Sensitivity:	95.3 pg/mL
Standard:	20000 pg/mL
Detection Range:	312.5-20000 pg/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Neuro science;
Uniprot ID:	Q9HC23
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PK2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
20000.00	0.199
10000.00	0.397
5000.00	0.557
2500.00	0.857
1250.00	1.152
625.00	1.565
312.50	1.762
0.00	2.115

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PK2 and the recovery rates were calculated by comparing the measured value to the expected amount of PK2 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-94%	90%
EDTA plasma(n=5)	81-93%	87%
Heparin plasma(n=5)	83-101%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.