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人丝蛋白Cγ(FLNC)酶联免疫吸附检测试剂盒

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ELK3057

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human FLNC(Filamin C Gamma) ELISA Kit
Reactivity:	Human
Alternative Names:	ABP-280; ABP280A; ABPA; ABPL; FLN2; Actin Binding Protein 280; ABP-280-like protein; Actin-binding-like protein; Filamin-2
Assay Type:	Sandwich
Sensitivity:	0.123 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Developmental science;
Uniprot ID:	Q14315
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLNC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLNC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLNC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLNC in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.146	2.060
10.00	1.674	1.588
5.00	1.119	1.033
2.50	0.828	0.742
1.25	0.498	0.412
0.63	0.321	0.235
0.32	0.221	0.135
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FLNC and the recovery rates were calculated by comparing the measured value to the expected amount of FLNC in samples.

Matrix	Recovery range	Average
serum(n=5)	78-95%	86%
EDTA plasma(n=5)	94-105%	98%
Heparin plasma(n=5)	85-97%	91%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FLNC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.