99499威尼斯(中国·WWW认证)游戏内容-Macau App Station

产品中心

人肉毒碱棕榈酰基转移酶2(CPT2)酶联免疫吸附检测试剂盒

ELK3217

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human CPT2(Carnitine Palmitoyltransferase 2, Mitochondrial) ELISA Kit
Reactivity:	Human
Alternative Names:	CPTASE; Carnitine O-Palmitoyltransferase 2; Carnitine palmitoyltransferase II
Assay Type:	Sandwich
Sensitivity:	0.112 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;Neuro science;
Uniprot ID:	P23786
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.219	2.136
10.00	1.587	1.504
5.00	1.142	1.059
2.50	0.831	0.748
1.25	0.535	0.452
0.63	0.297	0.214
0.32	0.206	0.123
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CPT2 and the recovery rates were calculated by comparing the measured value to the expected amount of CPT2 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-103%	96%
EDTA plasma(n=5)	78-96%	87%
Heparin plasma(n=5)	86-99%	92%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CPT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.