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人神经肽FF(NPFF)酶联免疫吸附检测试剂盒

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ELK3724

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human NPFF(Neuropeptide FF) ELISA Kit
Reactivity:	Human
Alternative Names:	FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF
Assay Type:	Competitive Inhibition
Sensitivity:	6.7 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	2h
Research Area:	Neuro science;
Uniprot ID:	O15130
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NPFF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	0.219
500.00	0.479
250.00	0.793
125.00	0.997
62.50	1.236
31.25	1.579
15.63	1.861
0.00	2.197

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NPFF and the recovery rates were calculated by comparing the measured value to the expected amount of NPFF in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	92-107%	99%
Heparin plasma(n=5)	78-92%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NPFF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.