99499威尼斯(中国·WWW认证)游戏内容-Macau App Station

产品中心

人孤腓肽(OFQ/N)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK4683

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human OFQ/N(OrphaninFQ/Nociceptin) ELISA Kit
Reactivity:	Human
Alternative Names:	PPNOC; Prepronociceptin; Pocistatin; Pro-nociceptin; Preproorphanin FQ; Neuropeptide 1; Nociceptin; Orphanin FQ; PNOC; Pronociceptin
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OFQ/N. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OFQ/N. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OFQ/N, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OFQ/N in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.058	1.969
5.00	1.616	1.527
2.50	1.121	1.032
1.25	0.883	0.794
0.63	0.593	0.504
0.32	0.326	0.237
0.16	0.288	0.199
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OFQ/N and the recovery rates were calculated by comparing the measured value to the expected amount of OFQ/N in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	97-105%	101%
Heparin plasma(n=5)	83-99%	95%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OFQ/N and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.