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人食欲素(OX)酶联免疫吸附检测试剂盒

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ELK4722

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview 文献(1)

Product name:	Human OX(Orexin) ELISA Kit
Reactivity:	Human
Alternative Names:	HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor
Assay Type:	Competitive Inhibition
Sensitivity:	4.6 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	serum, plasma, tissue homogenates and other biological fluids
Assay length:	2h
Research Area:	Neuro science;
Uniprot ID:	O43612
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OX in the samples is then determined by comparing the OD of the samples to the standard curve.

World Neurosurgery

Median Nerve Stimulation Attenuates Traumatic Brain Injury–Induced Comatose State by Regulating the Orexin-A/RasGRF1 Signaling Pathway

日期：2022-09-03 IF：2.21

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	0.183
500.00	0.310
250.00	0.527
125.00	0.898
62.50	1.289
31.25	1.439
15.63	1.835
0.00	2.475

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OX and the recovery rates were calculated by comparing the measured value to the expected amount of OX in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	85-95%	90%
Heparin plasma(n=5)	79-93%	91%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.