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人含溴区蛋白1(BRD1)酶联免疫吸附检测试剂盒

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ELK5006

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human BRD1(Bromodomain Containing Protein 1) ELISA Kit
Reactivity:	Human
Alternative Names:	BRL; BRPF2; BR140-like protein; Bromodomain and PHD finger-containing protein 2
Assay Type:	Sandwich
Sensitivity:	0.053 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample type:	Tissue homogenates, cell lysates and other biological fluids.
Assay length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	O95696
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.121	2.029
5.00	1.721	1.629
2.50	1.073	0.981
1.25	0.733	0.641
0.63	0.509	0.417
0.32	0.313	0.221
0.16	0.175	0.083
0.00	0.092	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant BRD1 and the recovery rates were calculated by comparing the measured value to the expected amount of BRD1 in samples.

Matrix	Recovery range	Average
serum(n=5)	92-106%	99%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-103%	95%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BRD1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.