99499威尼斯(中国·WWW认证)游戏内容-Macau App Station

产品中心

人前列腺素E受体2(EP2)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK5036

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human EP2(Prostaglandin E Receptor 2) ELISA Kit
Reactivity:	Human
Alternative Names:	PTGER2; Prostaglandin E2 Receptor(Subtype EP2),53kD
Assay Type:	Sandwich
Sensitivity:	30 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample type:	Tissue homogenates, cell lysates and other biological fluids.
Assay length:	3.5h
Research Area:	Signal transduction;Hormone metabolism;
Uniprot ID:	P43116
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.201	2.107
2500.00	1.672	1.578
1250.00	1.283	1.189
625.00	0.752	0.658
312.50	0.532	0.438
156.25	0.335	0.241
78.13	0.175	0.081
0.00	0.094	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant EP2 and the recovery rates were calculated by comparing the measured value to the expected amount of EP2 in samples.

Matrix	Recovery range	Average
serum(n=5)	96-107%	102%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	83-95%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.