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大鼠钙网蛋白(CRT)微量上样酶联免疫吸附检测试剂盒

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ELK6600MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Rat CRT(Calreticulin) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	CALR; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60
Assay Type:	Sandwich
Sensitivity:	14.3 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Signal transduction;Metabolic pathway;Bone metabolism;
Uniprot ID:	P18418
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CRT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CRT in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.153	2.068
1000.00	1.440	1.355
500.00	1.215	1.130
250.00	0.800	0.715
125.00	0.491	0.406
62.50	0.387	0.302
31.25	0.184	0.099
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CRT and the recovery rates were calculated by comparing the measured value to the expected amount of CRT in samples.

Matrix	Recovery range	Average
serum(n=5)	87-113%	100%
EDTA plasma(n=5)	90-96%	93%
Heparin plasma(n=5)	86-92%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.