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人防御素β124(DEFb124)微量上样酶联免疫吸附检测试剂盒

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ELK6684MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human DEFb124(Defensin Beta 124) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	DEFB-24
Assay Type:	Sandwich
Sensitivity:	6 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Immune molecule;
Uniprot ID:	Q8NES8
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEFb124. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFb124. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DEFb124, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DEFb124 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.152	2.051
500.00	1.670	1.569
250.00	1.220	1.119
125.00	0.856	0.755
62.50	0.501	0.400
31.25	0.379	0.278
15.63	0.202	0.101
0.00	0.101	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant DEFb124 and the recovery rates were calculated by comparing the measured value to the expected amount of DEFb124 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-96%	89%
EDTA plasma(n=5)	94-110%	102%
Heparin plasma(n=5)	90-101%	96%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFb124 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.