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人活化T-细胞核因子2(NFATC2)微量上样酶联免疫吸附检测试剂盒

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ELK6857MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human NFATC2(Nuclear Factor Of Activated T-Cells, Cytoplasmic 2) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	NFAT1; NFATP; NF-ATP; NFAT pre-existing subunit; T-cell transcription factor NFAT1
Assay Type:	Sandwich
Sensitivity:	5.7 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5h
Research Area:	Infection immunity;
Uniprot ID:	Q13469
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFATC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFATC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFATC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFATC2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.287	2.192
500.00	1.532	1.437
250.00	1.101	1.006
125.00	0.863	0.768
62.50	0.481	0.386
31.25	0.295	0.200
15.63	0.225	0.130
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NFATC2 and the recovery rates were calculated by comparing the measured value to the expected amount of NFATC2 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-103%	95%
EDTA plasma(n=5)	86-129%	108%
Heparin plasma(n=5)	88-99%	94%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFATC2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.