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大鼠可溶性半乳糖凝集素3结合蛋白(LGALS3BP)微量上样酶联免疫吸附检测试剂盒

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ELK7536MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Rat LGALS3BP(Lectin Galactoside Binding, Soluble 3 Binding Protein) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	MAC-2-BP; LGALS3-BP; Galectin-3-Binding Protein; L3 Antigen; Mac-2-Binding Protein; Tumor-associated antigen 90K; Serum Protein 90K; Basement membrane autoantigen p105
Assay Type:	Sandwich
Sensitivity:	0.68 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Infection immunity;Immunodeficiency;
Uniprot ID:	O70513
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LGALS3BP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LGALS3BP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LGALS3BP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LGALS3BP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	2.292	2.195
50.00	1.785	1.688
25.00	1.128	1.031
12.50	0.818	0.721
6.25	0.578	0.481
3.13	0.313	0.216
1.56	0.175	0.078
0.00	0.097	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LGALS3BP and the recovery rates were calculated by comparing the measured value to the expected amount of LGALS3BP in samples.

Matrix	Recovery range	Average
serum(n=5)	81-108%	95%
EDTA plasma(n=5)	82-99%	91%
Heparin plasma(n=5)	84-104%	94%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LGALS3BP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.