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脱氢表雄酮(DHEA)酶联免疫吸附检测试剂盒

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ELK7826

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(1)

Product name:	DHEA(Dehydroepiandrosterone) ELISA Kit
Reactivity:	General
Alternative Names:	DHEA
Assay Type:	Competitive Inhibition
Sensitivity:	47.8 pg/mL
Standard:	10000 pg/mL
Detection Range:	156.25-10000 pg/mL
Sample type:	Serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;Rheumatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Square

Analysis of prevalence and associated biomarkers of sarcopenia living in rural community older adults in Wuhan, China

日期：2024-08-20 IF：3.8

标准曲线

Concentration (pg/mL)	OD	Corrected OD
10000.00	0.191
5000.00	0.332
2500.00	0.567
1250.00	0.878
625.00	1.112
312.50	1.537
156.25	1.733
0.00	2.028

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant DHEA and the recovery rates were calculated by comparing the measured value to the expected amount of DHEA in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	90-103%	96%
Heparin plasma(n=5)	87-99%	98%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHEA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.