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叶酸(FA)酶联免疫吸附检测试剂盒

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ELK7834

规格：	价格：
48T	￥1680.00
96T	￥2400.00

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Overview 文献(1)

Product name:	FA(Folic Acid) ELISA Kit
Reactivity:	General
Alternative Names:	VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate
Assay Type:	Competitive Inhibition
Sensitivity:	14 pg/mL
Standard:	10000 pg/mL
Detection Range:	156.25-10000 pg/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	2h
Research Area:	Hematology;Reproductive science;Nutrition metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FA in the samples is then determined by comparing the OD of the samples to the standard curve.

peerj

RNA sequencing-based exploration of the effects of far-red light on microRNAs involved in the shade-avoidance response of D. officinale

日期：2023-03-20 IF：3.061

标准曲线

Concentration (pg/mL)	OD	Corrected OD
10000.00	0.212
5000.00	0.397
2500.00	0.583
1250.00	0.857
625.00	1.152
312.50	1.511
156.25	1.715
0.00	2.226

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FA and the recovery rates were calculated by comparing the measured value to the expected amount of FA in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	80-94%	87%
Heparin plasma(n=5)	89-101%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.