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1,5-脱水葡萄糖醇(1,5-AG)酶联免疫吸附检测试剂盒

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ELK7840

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	1,5-AG(1,5-Anhydroglucitol) ELISA Kit
Reactivity:	General
Alternative Names:	LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol
Assay Type:	Competitive Inhibition
Sensitivity:	0.6 µg/mL
Standard:	150 µg/mL
Detection Range:	2.35-150 µg/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (µg/mL)	OD	Corrected OD
150.00	0.175
75.00	0.356
37.50	0.672
18.75	0.913
9.38	1.253
4.69	1.587
2.35	1.799
0.00	2.101

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 1,5-AG and the recovery rates were calculated by comparing the measured value to the expected amount of 1,5-AG in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	84-102%	93%
Heparin plasma(n=5)	87-95%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 1,5-AG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.