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激素脱落酸(ABA)酶联免疫吸附检测试剂盒

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ELK7842

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	ABA(Abscisic Acid) ELISA Kit
Reactivity:	General
Alternative Names:	AB-A; abscisin II; Dormin
Assay Type:	Competitive Inhibition
Sensitivity:	0.53 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample type:	tissue or cell culture supernates
Assay length:	2h
Research Area:	Metabolic pathway;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ABA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ABA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	0.191
50.00	0.275
25.00	0.446
12.50	0.531
6.25	0.977
3.13	1.201
1.57	1.813
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ABA and the recovery rates were calculated by comparing the measured value to the expected amount of ABA in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	87-99%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ABA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.