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人抗生长激素抗体(Anti-GH)酶联免疫吸附检测试剂盒

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ELK7847

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human Anti-GH(Anti-Growth Hormone Antibody) ELISA Kit
Reactivity:	Human
Alternative Names:	GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal
Assay Type:	Competitive Inhibition
Sensitivity:	1.07 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample type:	Serum, plasma and other biological fluids.
Assay length:	2.5h
Research Area:	Endocrinology;Neuro science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-GH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GH in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
200.00	0.227
100.00	0.386
50.00	0.575
25.00	0.878
12.50	1.169
6.25	1.537
3.13	1.796
0.00	2.256

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-GH and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-GH in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	80-95%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-GH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.