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3-硝基酪氨酸(3-NT)酶联免疫吸附检测试剂盒

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ELK7866

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(1)

Product name:	3-NT(3-Nitrotyrosine) ELISA Kit
Reactivity:	General
Alternative Names:	3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine
Assay Type:	Competitive Inhibition
Sensitivity:	1.4 ng/mL
Standard:	300 ng/mL
Detection Range:	4.69-300 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Metabolic pathway;Infection immunity;Rheumatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.

Indian Journal of Clinical Biochemistry

Elevated Oxidized LDL Level is Associated with Systematic Coronary Risk Evaluation (SCORE2) High-Risk Prediction Algorithm: A Preliminary Study

日期：2024-06-26 IF：1.5

标准曲线

Concentration (ng/mL)	OD	Corrected OD
300.00	0.217
150.00	0.467
75.00	0.793
37.50	0.967
18.75	1.236
9.38	1.596
4.69	1.861
0.00	2.265

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.

Matrix	Recovery range	Average
serum(n=5)	92-107%	99%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	78-92%	84%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.