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25-羟基维生素D3(HVD3)酶联免疫吸附检测试剂盒

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ELK7890

规格：	价格：
48T	￥1680.00
96T	￥2400.00

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Overview 文献(1)

Product name:	HVD3(25-Hydroxyvitamin D3) ELISA Kit
Reactivity:	General
Alternative Names:	25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
Assay Type:	Competitive Inhibition
Sensitivity:	0.91 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Obesity Medicine

Impact of hypovitaminosis D on body weight, food intake parameters and hematological alterations in albino mice Mus musculus

日期：2022-11-07 IF：-

标准曲线

Concentration (ng/mL)	OD	Corrected OD
200.00	0.179
100.00	0.334
50.00	0.524
25.00	0.827
12.50	1.124
6.25	1.572
3.13	1.755
0.00	2.101

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HVD3 and the recovery rates were calculated by comparing the measured value to the expected amount of HVD3 in samples.

Matrix	Recovery range	Average
serum(n=5)	92-106%	99%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-103%	86%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HVD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.