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羧甲基赖氨酸(CML)酶联免疫吸附检测试剂盒

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ELK7896

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(1)

Product name:	CML(Carboxymethyl Lysine) ELISA Kit
Reactivity:	General
Alternative Names:	N(6)-Carboxymethyllysine
Assay Type:	Competitive Inhibition
Sensitivity:	24.7 ng/mL
Standard:	5000 ng/mL
Detection Range:	78.13-5000 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Metabolic pathway;Endocrinology;Cardiovascular biology;Genetic science;Nutrition metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CML. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CML. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CML in the samples is then determined by comparing the OD of the samples to the standard curve.

Cureus Journal of Medical Science

Assessment of Visual Evoked Potential to Detect Visual Pathway Dysfunction in Gestational Diabetes Mellitus: A Longitudinal Case-Control Study With Post-partum Follow-up

日期：2023-11-24 IF：1.2

标准曲线

Concentration (ng/mL)	OD	Corrected OD
5000.00	0.191
2500.00	0.275
1250.00	0.451
625.00	0.846
312.50	1.389
156.25	1.439
78.13	1.761
0.00	2.475

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CML and the recovery rates were calculated by comparing the measured value to the expected amount of CML in samples.

Matrix	Recovery range	Average
serum(n=5)	80-93%	86%
EDTA plasma(n=5)	94-105%	98%
Heparin plasma(n=5)	95-107%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CML and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.