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炔雌醇(EE)酶联免疫吸附检测试剂盒

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ELK8055

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	EE(Ethinylestradiol) ELISA Kit
Reactivity:	General
Alternative Names:	Ethinyl Estradiol ; Ethynyl Estradiol; Ethinyl Oestradiol
Assay Type:	Competitive Inhibition
Sensitivity:	9.51 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with EE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EE in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	0.197
1000.00	0.385
500.00	0.596
250.00	0.823
125.00	1.157
62.50	1.583
31.25	1.624
0.00	2.112

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant EE and the recovery rates were calculated by comparing the measured value to the expected amount of EE in samples.

Matrix	Recovery range	Average
serum(n=5)	83-99%	95%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	85-97%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.