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人抗甘露糖结合蛋白抗体(Anti-MBL)酶联免疫吸附检测试剂盒

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ELK8057

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit
Reactivity:	Human
Alternative Names:	MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Assay Type:	Sandwich
Sensitivity:	2.54 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample type:	Serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Signal transduction;Infection immunity;Immune molecule;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
400.00	2.126	2.037
200.00	1.674	1.585
100.00	1.174	1.085
50.00	0.912	0.823
25.00	0.548	0.459
12.50	0.305	0.216
6.25	0.156	0.067
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-MBL and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-MBL in samples.

Matrix	Recovery range	Average
serum(n=5)	85-99%	92%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	96-107%	102%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-MBL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.