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人杀菌肽LL-37(LL-37)微量上样酶联免疫吸附检测试剂盒

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ELK8189MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human LL-37(Antibacterial Protein LL-37) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
Assay Type:	Sandwich
Sensitivity:	0.28 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
50.00	2.040	1.957
25.00	1.561	1.478
12.50	1.015	0.932
6.25	0.875	0.792
3.13	0.517	0.434
1.56	0.337	0.254
0.78	0.226	0.143
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-109%	99%
EDTA plasma(n=5)	94-113%	104%
Heparin plasma(n=5)	93-113%	103%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.