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小鼠线粒体开放阅读框12S rRNA-C(MOTS-c)酶联免疫吸附检测试剂盒

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ELK8232

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Mouse MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
Reactivity:	Mouse
Alternative Names:	MOTS-c
Assay Type:	Competitive Inhibition
Sensitivity:	0.87 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	2h
Research Area:	Developmental science;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MOTS-c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
200.00	0.211
100.00	0.467
50.00	0.675
25.00	0.967
12.50	1.157
6.25	1.565
3.13	1.861
0.00	2.132

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MOTS-c and the recovery rates were calculated by comparing the measured value to the expected amount of MOTS-c in samples.

Matrix	Recovery range	Average
serum(n=5)	84-106%	100%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	87-93%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOTS-c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.