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大鼠前心钠肽(Pro-ANP)微量上样酶联免疫吸附检测试剂盒

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ELK8360MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Rat Pro-ANP(Pro Atrial Natriuretic Peptide) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	Pro-ANP
Assay Type:	Sandwich
Sensitivity:	15.2 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Uniprot ID:	P01161
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
4000.00	2.150	2.064
2000.00	1.661	1.575
1000.00	1.120	1.034
500.00	0.851	0.765
250.00	0.538	0.452
125.00	0.316	0.230
62.50	0.251	0.165
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Pro-ANP and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ANP in samples.

Matrix	Recovery range	Average
serum(n=5)	90-102%	96%
EDTA plasma(n=5)	84-97%	91%
Heparin plasma(n=5)	93-120%	107%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.