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小鼠4-羟基壬烯酸(4-HNE)微量上样酶联免疫吸附检测试剂盒

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ELK8374MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Mouse 4-HNE(4-Hydroxynonenal) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal
Assay Type:	Sandwich
Sensitivity:	1.1 ng/mL
Standard:	150 ng/mL
Detection Range:	2.35-150 ng/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
150.00	1.979	1.889
75.00	1.828	1.738
37.50	1.309	1.219
18.75	0.868	0.778
9.38	0.593	0.503
4.69	0.331	0.241
2.34	0.197	0.107
0.00	0.090	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.

Matrix	Recovery range	Average
serum(n=5)	85-101%	93%
EDTA plasma(n=5)	94-112%	103%
Heparin plasma(n=5)	90-107%	99%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.