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大鼠白介素1β前体(pro-IL1b)微量上样酶联免疫吸附检测试剂盒

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ELK8404MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Rat pro interleukin 1 beta (pro-IL1b) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β
Assay Type:	Sandwich
Sensitivity:	16.7 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
4000.00	1.976	1.872
2000.00	1.527	1.423
1000.00	0.986	0.882
500.00	0.809	0.705
250.00	0.551	0.447
125.00	0.312	0.208
62.50	0.258	0.154
0.00	0.104	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant pro-IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of pro-IL1b in samples.

Matrix	Recovery range	Average
serum(n=5)	92-107%	100%
EDTA plasma(n=5)	87-126%	107%
Heparin plasma(n=5)	91-99%	95%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pro-IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.