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人磷酸化Tau蛋白(pMAPT/pTAU)酶联免疫吸附检测试剂盒

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ELK8412

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Human pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Reactivity:	Human
Alternative Names:	pMAPT /pTAU
Assay Type:	Sandwich
Sensitivity:	12.98 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample type:	serum, plasma, other biological fluids
Assay length:	3.5h
Research Area:	intracellular signal transduction and neuron's development and viability.
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.195	2.109
1000.00	1.685	1.599
500.00	1.128	1.042
250.00	0.866	0.780
125.00	0.539	0.453
62.50	0.313	0.227
31.25	0.221	0.135
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant pMAPT /pTAU and the recovery rates were calculated by comparing the measured value to the expected amount of pMAPT /pTAU in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	83-97%	90%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pMAPT /pTAU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.