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小鼠配对框基因6(PAX6)微量上样酶联免疫吸附检测试剂盒

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ELK8426MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Mouse PAX6(Paired Box Gene 6) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	AN; AN2; MGDA; WAGR; Oculorhombin; Aniridia,Keratitis; Aniridia type II protein
Assay Type:	Sandwich
Sensitivity:	25.7 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	P63015
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAX6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAX6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAX6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAX6 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.072	1.977
2500.00	1.679	1.584
1250.00	1.210	1.115
625.00	0.821	0.726
312.50	0.598	0.503
156.25	0.336	0.241
78.13	0.201	0.106
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PAX6 and the recovery rates were calculated by comparing the measured value to the expected amount of PAX6 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-113%	97%
EDTA plasma(n=5)	95-110%	103%
Heparin plasma(n=5)	94-116%	105%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAX6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.